Therapeutic proteins have revolutionized the treatment of many severe diseases like multiple sclerosis, haemophilia, diabetes and many more. Unfortunately, even recombinant proteins with structures (almost) identical to those of their endogenous counterparts often induce the formation of anti drug antibodies…

PURPOSE: To monitor the biodistribution of IgG1 aggregates upon subcutaneous (SC) and intravenous (IV) administration in mice and measure their propensity to stimulate an early immune response.

This thesis focuses on amyloid proteins, a class of proteins that convert into amyloid fibrils.

PURPOSE: Determine the effect of minute quantities of sub-visible aggregates on the in vitro immunogenicity of clinically relevant protein therapeutics.

Developing robust and comprehensive analytical methods to size and quantify subvisible particles in biopharmaceutical products.

Read-Urban was a first project to investigate whether policy recommendations can be made with the aid of linked data collections and data science and to gain experience with the success factors for such a process.

Oxidation is a common degradation pathway that affects therapeutic proteins and peptides during production, purification, formulation, transportation, storage and handling of solid and liquid preparations. In the present work we review the scientific literature about structural and biological consequences…

Promotors: W. Jiskoot; G.J. Witkamp, Co-promotor: M.R. Nejadnik

Promotor: Prof.dr. J.P. Abrahams

Promotor: G.P. van Wezel

How presence, abundance and enzymatic activities of AM and EM fungi differently affect soil carbon sequestration processes?

How soil microbial composition impacts structure and composition of the aboveground plant and animal communities?

Promotor: W. Jiskoot, Co-promotor: A. Hawe

Development of an aggregated impact indicator to measure decoupling.

Master projects are available in

Particles are omnipresent in biopharmaceutical products. In protein-based therapeutics such particles are generally associated with impurities, either derived from the drug product itself (e.g. protein aggregates), or from extrinsic contaminations (e.g. cellulose fibers).

While colloidal aggregates are often undesirable due to their random shapes, we exploit them as a starting point to synthesize patchy particles.

Promotor: W. Jiskoot, Co-promotor: H.A. Every

Solar energy is used by photosynthetic organisms to drive energy required cellular processes. Is absorbed by two groups of pigments, located in the LHCs.

Beowulf clusters looking for new frontiers.

SMR can be used to measure the mass of adsorbed protein to nanoparticles with a high precision in the presence of free protein.

Mouse models are commonly used to assess the immunogenicity of therapeutic proteins and to investigate the immunological processes leading to antidrug antibodies. The aim of this work was to develop a transgenic (TG) Balb/c mouse model for evaluating the immunogenicity of recombinant human insulin (insulin)…

Firearms and violence in Europe–A systematic review by Katharina Krüsselmann, Pauline Aarten, Marieke Liem

Indicators to measure progress in de-linking economic growth and environmental pressure.

The objective is to develop in vitro antigen degradation assays in order to mimic the fate of the antigen in vivo.

Article in the journal Irish Political Studies discussing the challenge of aggregating opinion polls and presenting a method to better model major sudden political and societal events. This can can enhance opinion poll reporting in the media.

Can we exploit the cell death machinery of the host to develop new host-directed anti-TB treatments?

Our group operates at the cross-roads of different disciplines: molecular and cellular microbiology, biochemistry, structural biology and biophysics. The group harbors expertise in a multitude of molecular and cellular approaches including:

The project falls in the context of the EU Thematic Strategy on the Sustainable Use of Natural Resources (COM(2005)670). The Institute for Environment and Resources (IES) of the Joint Research Centre (JRC) has developed three sets of decoupling indicators. Such indicators require the definition of a…

This article argues that, given the current pervasive uncertainty about the reliability of jury deliberation, we ought to treat it with epistemic humility.

Promotor: Prof.dr. E.F. Smets, Co-promotores: Dr. B. Gravendeel, F. Pupulin

This paper provides an initial answer to the question regarding eco-labels: ‘How can we make the development of eco-labels more structured and transparent?’

Parkinson's disease is the second most common neurodegenerative disease in the world. One of its symptoms is the loss of dopaminergic neurons in the substantia nigra pars compacta.

The aim of this thesis is to develop and study a robust and adaptable scaffold for supramolecular polymer self-assembly in water.

The thesis describes the use of electron paramagnetic resonance (EPR) spectroscopy, in continuous wave and pulse modes, to address the interaction of α-Synuclein (αS) with membranes and the aggregation of αS.

Novel entities may pose risks to humans and the environment. The small particle size and relatively large surface area of micro- and nanoparticles (MNPs) make them capable of adsorbing other novel entities, leading to the formation of aggregated contamination.

The ReCiPe methodology for Life Cycle Impact Assessment (LCIA).

The high prevalence of pure red cell aplasia in Thailand has been associated with the sharp increase in number of recombinant human erythropoietin (rhEPO) copy products, based on a classical generic regulatory pathway, which have entered the market.

Macromolecular Biochemistry is a section of the Leiden Institute of Chemistry at Leiden University, comprising the PIs Marcellus Ubbink, Remus Dame, Aimee Boyle, Lars Jeuken and Anne Wentink.

Mining time series is a machine learning subfield that focuses on a particular data structure, where variables are measured over (short or long) periods of time.

Promotor: T. Schmidt

In this work, we attempt to answer the question:

This thesis studied in depth the energy use and CO2 emissions of the industrial sector in China.

TRANSAUTOPHAGY is a European COST action to promote multidisciplinary research on autophagy and translation of knowledge to applications.

Decentralized plug and play systems for energy production are the future picture of our society. Artificial photosynthetic systems are used for this purpose.

Honorata Mazepus and Jaroslaw Kantorowicz are assistant professors at the Institute of Security and Global Affairs. Mazepus and Kantorowicz are one of the authors of this article in Nature on the role of national identity on public health support during global pandemics.