Pericytes, the mural cells of blood microvessels, are important regulators of vascular morphogenesis and function that have been postulated to mechanically control microvascular diameter through as yet unknown mechanisms.

Parkinson's disease is the second most common neurodegenerative disease in the world. One of its symptoms is the loss of dopaminergic neurons in the substantia nigra pars compacta.

Promotor: R. Tijdeman, Co-promotor: C. Kraaikamp

The aim of this work was to study the applicability of antigen-coated pH-sensitive microneedle arrays for effective vaccination strategies. Therefore, a model antigen (ovalbumin) was coated onto pH-sensitive (pyridine-modified) microneedle arrays to test pH-triggered antigen release by applying the…

Dendritic cells (DC) play a prominent role in the priming of CD8(+) T cells. Vaccination is a promising treatment to boost tumor-specific CD8(+) T cells which is crucially dependent on adequate delivery of the vaccine to DC.

Chemical-induced organ toxicity is a major concern in the development and use of chemicals, including drugs, pesticides, industrial chemicals and cosmetics. In the last decades, the onset and progression of chemical-induced organ toxicity has been linked amongst others to perturbation of mitochondri…

Synthetic hydrogels that mimic the natural extracellular matrix in the biophysical and biochemical cues it provides to cells are in high demand, however the cell phenotypes as they are observed in vivo in numerous cases have yet to be attained.

The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.

Promotor: Prof.dr. B. van de Water, Co-promotor: L.S. Price

Mechanical forces are known to play an important role in bacterial colonies. In this dissertation, we study the self-organization at various stages of growing bacterial colonies, and focus on the mechanical effects of cell growth.

A research goal for ABS is to develop miniaturized platforms for research in (stem)cell, in-vitro systems and application in clinical and preventive research. Combined with the high throughput, this development will make it possible to study the dynamics of pathogenesis in human and cellular models…

During the development of the (human) body from a single fertilized egg, organs and structures must form. The development and growth of biological forms is called morphogenesis.

This thesis aims to test in vitro pipelines to rapidly and cost-effectively identify molecular mechanisms that drive adverse outcomes in clinical use.

The way organisms develop from the initial single-cellular state to a complex final assembly like the human body, and how the final body is maintained throughout life, is one of the greatest mysteries and it’s understanding one of the biggest scientific challenges.

Promotor: Prof.dr. J. Kuiper, Co-Promotor: S.C.A. de Jager

Promotor: B. van de Water

Promotor: P. J. J. Hooykaas, Co-promotor: R. Offringa

This study explores the role of linguistic data in the reconstruction of Dolgan (pre)history by analyzing contact-induced changes and using them to infer information about the nature of the contact settings in which they occurred.

Atherosclerosis, in particular rupture of atherosclerotic plaques, is the underlying cause of most heart attacks or strokes and is the leading cause of death worldwide.

Atherosclerosis is the main underlying pathology of cardiovascular disease. Atherosclerosis is caused by an immune response which is directed against (modified) lipoproteins which accumulate in the vessel wall.

Source: Mediators Inflamm (2014)

Promotoren: G.F.A. Kersten, W. Jiskoot, Co-promotor: J-P. Amorij

Bacteria have the ability to alter their morphology in order to adapt to changing environments.

Despite being the object of intense study, embryonic development has been difficult to model due to a number of reasons. First, complex tissues can be comprised of many cell types, of which we probably only know a subset.

The single cell group (SCG) is part of the Metabolomics & Analytics Centre Our research is centered around developing innovating analytical methods tailored for small-volume, or single cell analysis to enable metabolomics-driven systems biology in personalized health strategies. We are always on…

In this thesis, single-cell approaches were applied to characterize the impact of aging on the immunological B cell landscape in different models of atherosclerotic cardiovascular disease, with the goal to identify and study new B cell-associated biomarkers and therapeutic targets to halt atherosclerosis…

The first steps are made in personalised cancer vaccination strategies, which aim to induce a more specific immune response with fewer side effects. The activation of cytotoxic T cells is crucial for an effective immune response.

Drug induced organ toxicity is the main problem of the drug development and drug usage in the clinic. The liver and kidneys are the most sensitive organs towards drug induced toxicity.

Synthetic or artificial cells are vesicles, often lipid vesicles, with wide ranging applications from drug delivery to nanoreactors in biocatalysis. We are developing polymer-lipid hybrid vesicles for applications in compartmentalised biocatalysis, in which the polymer has been shown to greatly enhance…

Atherosclerosis is the most prominent underlying pathology of cardiovascular disease and an important cause of major adverse cardiovascular events.

We have studied the impact of particle shape anisotropy, multivalent interactions and flexibility on systems of micron-sized colloidal particles.

Leiden has a long history in the treatment of blood cell cancer. Research to find better therapies never stands still. One of the potential treatments currently being worked on is a ‘living medicine’.

Promotor: Prof.dr. T. Schmidt, Co-promotor: Dr. ir. S. J.T. van Noort

Promotor: Prof.dr. J. Kuiper

In this thesis, we describe the potential of cell mechanical phenotyping for immune cell characterization, diagnosis, drug testing, as well as treatment of disease.

Mechanical interactions between cells and their environment play an important role in many biological processes.

The bacterial cell wall is a nearly universal structure that offers protection and gives the cell its shape. However, environmental stressors, such as cell wall-targeting antibiotics and hyperosmotic conditions, can induce bacteria to shift to a wall-deficient state. It is unknown whether the lack of…

What distinguishes a tumour cell from a healthy cell? Researchers are trying to answer this question as precisely as possible. Certain differences could eventually lead to new therapies.

The CMCB brings together microbial cell biology expertise in Leiden. It aims at optimal resonance between the groups and maximal exploitation of the available infrastructure and to develop innovative cross-boundary technology platforms.

The LUMC iPSC Hotel offers the generation of research grade human induced pluripotent stem cells (hiPSCs) from different tissue sources using state of the art reprogramming techniques.

Cardiovascular disease is a major global burden and atherosclerosis is the main underlying pathological process.

Promotor: J. Aarts

Almost all bacteria are enveloped by a cell wall that provides cellular protection.

Gaucher disease is a rare metabolic disorder, in which a mutation of the enzyme glucocerebrosidase (GBA1) causes buildup of its substrate glucosylceramide and the deacylated counterpart glucosylsphingosine in lysosomes.

Promotor: R.C.M. Pelger Co-Promotor: G. van der Pluijm

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

Cell-based medicinal products (CBMPs), such as CAR T cells, are highly complex biopharmaceutical products offering various treatment options for serious illnesses such as cancer or autoimmune diseases.