This thesis explores squaramide-based supramolecular polymers and hydrogels, enhancing their mechanical strength for 3D cell culture.

Heterogeneous catalyzed processes are highly significant to the chemical industry. Dissociative chemisorption (DC) of molecules on surfaces is always considered as a step with a high degree of rate control for heterogeneous catalysis.

Multicopper oxidase, laccase, can efficiently reduce oxygen to water and are mostly used in the enzymatic biofuel cells.

Promotor: J.G.E.M.Fraaije, Co-promotor: A. Kros

Promotor: G.A. van der Marel, Co-promotor: J.D.C. Codée

Advanced sensing techniques require graphene with high quality and well-controlled surface chemistry.

This dissertation contains four works during my PhD. Different biomaterials have been designed based on coiled-coil peptides. These biomaterials have a range of applications, inclusing drug delivery, cell sorting to cell-cell fusion.

This doctoral thesis is an effort to understand how lipid phase-separation induced by diacylglycerol analogues in lipid-based nanoparticles affects their in vivo behavior, leading to specific nanoparticle-protein communications and selective cell targeting.

A structural investigation of coiled coil peptides used as membrane fusogens, mimicing naturally occuring coiled-coil fusion proteins. Synthetic modifications have been made to alter lipid attachment, secondary structure and to insert photoactive azobenzene moieties for active control over coiled coil…

To design the ideal water oxidation catalyst, understanding of the catalytic mechanism and decomposition pathways is essential.

Indonesia is experiencing various environmental challenges related to its fast economic growth. Therefore, it is necessary to have measurable and applicable indicators to obtain accurate data and information regarding the costs of adverse environmental impacts arising from economic activities to support…

Hass

Understanding the formation and preservation of Pleistocene fire traces through laboratory-based experimental research

Our educational team has been awarded an ICTO-grant of €12000 for the LACDR for flipping the Biochemistry 2 classroom.

Although it is often assumed that the stereochemistry in Ugi multicomponent reactions is determined in the final Mumm rearrangement step, experimental and computational evidence that Ugi reactions on hydroxylated pyrrolines proceed under kinetic control is reported.

The role of the interfacial environment during electrochemical reactions has been increasingly valued, especially for those reactions involving protons or hydroxyl ions.

Promotor: M.T.M. Koper, Co-Promotor: L.B.F. Juurlink

PNAS publication on protein encounter complexes

Advanced UHF MRI techniques provide powerful tools for studying the zebrafish brain, an emerging model in neurological research. In this work, various MRI and localized MRS methods are optimized and employed for UHF strength for studying the zebrafish brain.

Monoacylglycerol lipase (MAGL) is the principal enzyme responsible for hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-AG). MAGL inhibition provides several potential therapeutic opportunities, including anti-nociceptive, anti-inflammatory and anti-cancer activity.

The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.

Ice, the solid state of water, plays an important role on our planet as well as the entire universe.Despite the fact that an individual water molecule has a very simple structure, its chemical bonding in the solid phase can be surprisingly complex.

Promotor: Prof.dr. M. Ubbink

This thesis describes the use of a combined approach of computational and experimental techniques to gain novel insights to understand the glycosylation reaction and its reactive intermediates.

This thesis describes the development and optimization of the first molecular tools to study the enzyme PLA2G4E.

Nucleoid associated proteins maintain the architecture of the bacterial chromosome and regulate gene expression, hinting that their role as transcription factors may involve local three-dimensional chromosome re-modelling.

The research aims to explore the evolutionary adaptability of enzymes and the impact of temperature on protein evolution pathways, using M. tuberculosis β-lactamase BlaC as the object of study. Enzymes inherently embody a delicate balance between activity and stability, and the acquisition of new enzymatic…

The studies presented in the work show the potential of the integrative use of biophysical data in defining the structural basis of protein interactions.

Promotor: M. Ubbink, Co-promotor: G. Siegal

Glycoside hydrolases (glycosidases/GHs) are widely abundant enzymes in all kingdoms of life and are important biocatalysts that catalyze the hydrolysis of glycosidic linkages in oligo/polysaccharides, glycoproteins and glycolipids with tremendous efficiency

This dissertation focused on computational methods based on first principles calculations using the Density Functional Theory (DFT) framework. Emphasis was laid on affordable methods that can provide a tradeoff between computational expense and accuracy.

As a virtually inexhaustible source, solar energy plays a major role in future global energy scenarios.

This perspective addresses four challenges facing theorists whose aim is to make quantitatively accurate predictions for reactions of molecules on metal surfaces, and suggests ways of meeting these challenges, focusing on dissociative chemisorption reactions of H2, N2, and CH4.

Pancreatic ductal adenocarcinoma (PDAC) is the deadliest common cancer, with only 8% of patients surviving for five years. One reason for this poor outcome is that current chemotherapy treatments are highly toxic, often leaving patients too sick to receive treatment or forcing doctors to lower the dose…

Promotores: Prof.dr. H.J.M. de Groot, Prof.dr. J. Matysik (Universitaet Leipzig)

Promotor: Prof.dr. G.J. Kroes

Promotor: E. Bouwman, Co-Promotor: S. Bonnet

To this day, all cyclophellitol-based inhibitors and ABPs have been close analogues of their natural substrate counterparts. As a result, these probes showed high selectivity towards their target glycosidases.

Promotor: E. Bouwman, Co-promotor: S. Bonnet

The glycosylation reaction is a pivotal reaction in creating new and complex oligosaccharides.

Promotor: E. Bouwman, Co-promotor: S. Bonnet

Glycoconjugate vaccines are composed of microbial poly- or oligosaccharides covalently linked to a carrier protein.

Glycosidases are essential in fundamental biological processes and are responsible for the degradation of most (oligo)saccharides, glycolipids and glycoproteins.

The research described in this Thesis was aimed at designing and synthesizing nature-inspired compounds as part of TB vaccine discovery.

This thesis systematically analyzes the physical-chemistry of lipid-graphene interactions with the major objective of reconciliating the variety of results reported in the literature.

ChEMBL is a large-scale drug discovery database containing bioactivity information primarily extracted from scientific literature.

Supervisor: Gerard van Westen

Promotor: G. A. van der Marel, Co-promotor: D. V. Filippov