Now online in Molecular Microbiology our studies on the motility of Sulfolobus acidocaldarius and its unique swimming organelle, the archaellum.

Authoritative and cutting-edge, Bacterial Chromatin: Methods and Protocols aims to be useful as an up-to-date reference work for scholars in the bacterial chromatin field, those entering the field from adjacent research fields, and scientists in the eukaryotic chromatin field.

The research presented in this thesis explores the chemotherapeutic potential of metal-based compounds as chemotherapy agents, with an initial focus on the synthesis and DNA interaction studies of platinum and palladium compounds utilizing the [Pt(bapbpy)]2+ scaffold. The study identifies intercalation…

Authoritative and cutting-edge, Bacterial Chromatin: Methods and Protocols, Second Edition aims to be a useful up-to-date reference work for researchers currently in the field and to those entering the field.

Promotor: M. Ubbink, Co-Promotor: R. T. Dame

Throughout all domains of life, from bacteria and archaea to eukaryotes, genomes adopt well-organized three-dimensional structures that can change in space and time to accommodate preferred transcriptional programs for environmental adaptation, the maintenance of cellular identity and differentiation…

All life on earth contains DNA, which is used to store biological information. Organisms compact their DNA in order for it to fit inside their cell(s).

Promotor: Prof.dr. J. Brouwer, Co-promotor: Dr. Remus Th. Dame

Nucleoid associated proteins maintain the architecture of the bacterial chromosome and regulate gene expression, hinting that their role as transcription factors may involve local three-dimensional chromosome re-modelling.

Every organisms in the tree of life faces the same challenge: the length of its DNA exceeds the volume of the cell it needs to fit in. Several strategies have evolved to solve this problem, one of them being the expression of proteins that bind and organize the DNA.

Several single-stranded RNA viruses make use of Xrn1-resistant RNAs in their 3’ untranslated regions of their genome RNAs in order to increase their pathogenicity.

Macromolecular Biochemistry is a section of the Leiden Institute of Chemistry at Leiden University, comprising the PIs Marcellus Ubbink, Remus Dame, Lars Jeuken, Anne Wentink, Anjali Pandit, René Olsthoorn, Alia Matysik, Sebastian Geibel and Steffen Brünle.

The CMCB brings together microbial cell biology expertise in Leiden. It aims at optimal resonance between the groups and maximal exploitation of the available infrastructure and to develop innovative cross-boundary technology platforms.

Bacteria often experience external challenges, such as changes in environmental conditions or attacks by bacteriophages.

Research at the Macromolecular Biochemistry group is comprised of the following research themes:

The CIGR brings together a diverse range of unique expertise in genome research rooted in biology, chemistry and physics. Members of the CIGR investigate genome folding and genome transactions. An important aspect is direct as well as long term relevance for medicine.

Research projects for students of the University of Leiden and other Dutch Universities are often available at the MacBio group. The research lines are also described in the Research section. For details of specific projects contact the supervisor (Ubbink, Dame, Boyle, Jeuken or Wentink).

With Sylvestre Bonnet, Jeroen Codée and Remus Dame, the Leiden Institute of Chemistry (LIC) will be getting no less than three new professors. Bonnet will become professor in Bioinorganic Chemistry, Codée professor in Organic Chemistry and Dame professor in Molecular and Cellular Biochemistry.

The intestinal bacterium E. coli can adapt to changes in its surroundings. Leiden scientists have discovered how the H-NS protein makes this possible. This new knowledge can be an important starting point in combatting bacteria and diseases such as peritonitis. Publication 2 October in the journal e…

An overview of awards and prizes granted to our staff and students in 2020, as well as special appointments at Leiden University and other institutions.

Dutch researchers have developed a new tool to label DNA for studying chromosomes in live cells. The tool is non-invasive and can be applied in culture but also in living organisms, such as zebrafish embryos. The team published their findings in the journal Nucleic Acids Research.

Mapping of bacterial genomes to combat infectious diseases

Seven Leiden researchers have been awarded a prestigious Vici grant by the Netherlands Organisation for Scientific Research (NWO).

A group of single-celled organisms organises its DNA in a similar way to higher organisms such as plants, animals, and fungi. However, the way packaged DNA is read out differs between the two related groups, Bram Henneman discovered. PhD defence on 5 December.

The team of Alireza Mashaghi at the Leiden Academic Centre for Drug Research has found a way to determine and classify the shape of proteins. Their new theory defines the topology of proteins as a simple and precise barcode that allows the identification of all types of folds. ‘This barcode enables…

In 2020, many of our staff and students have again won prestigious prizes and been awarded important research subsidies.