The Microscopy Unit houses, maintains and coordinates most of the microscopy equipment of the IBL. The available equipment ranges from conventional light and fluorescence microscopes, to confocal laser scanning and electron microscopes. In addition, infrastructure is available for histology, including…

In this Ph.D. thesis we study the interaction of low energy electrons with thin materials, namely layered materials (graphene, hexagonal boron nitride, molybdenum disulfide) and organic films. At these low energies the quantum mechanical wavelength of the electron wave function is in the order of a…

Promotor: T. Schmidt

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.

This is a yearly event where we move in one week from the physics of light through the composition of the microscope, fluorescence microscopy and end up with some image analysis All the lectures are backed by practicals to really put your obtained knowledge into practice

This thesis presents insights from our study of various correlated electron systems with a scanning tunneling microscope (STM). In ordinary metals, electron-electron interactions exist, but get substantially screened due to the sheer number of electrons.

Electron microscopy has become an extremely important techniquein a wide variety of elds.

The aim of this facility is to accelerate biomedical research by providing access to advanced light and electron microscopy and by sharing expertise about sample preparation, imaging modalities, data storage and image analysis.

This thesis described the development of novel scanning tunneling microscopy techniques to investigate strongly correlated electronic states in quantum matter.

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

In surface science there is great effort to move from studying simple, flat model surfaces in vacuum to investigating more complex model catalysts in gas environments (in situ). This thesis gives three examples of such studies using microscopy and spectroscopy.

Promotor: Prof.dr. J.M. van Ruitenbeek, Prof.dr. R.M. Tromp

In this thesis, we develop a novel technique called Optical Near-field Electron Microscopy (ONEM), which aims to combine the advantages of both optical and electron microscopy: the high resolution of electron microscopy and the low sample damage of optical microscopy.

In this thesis, we describe the latest advances in SQUID-detected Magnetic Resonance Force Microscopy (MRFM).

In this thesis we use Josephson and noise scanning tunneling microscopy for the study of conventional, unconventional (iron-based) and disordered superconductors. On the one hand, Josephson scanning tunneling microscopy allows us to directly visualize the superfluid density with high spatial resolut…

In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.

Intrigued by the way cells autonomously regulate their fate, we strive to understand and visualize cellular processes as the basis of Cell Signaling.

The dissertation describes TEM experiments on heterogeneous catalysts.

Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn, Co-promotor: Dr. M.J.M. Schaaf

n this PhD thesis, the recombination of different atomic lattices in stacked 2D materials such as twisted bilayer graphene is studied. Using the different possibilities of Low-Energy Electron Microscopy (LEEM), the domain forming between the two atomic layers with small differences is studied.

Promotores: Prof.dr. C.U. Keller, Prof.dr. H.C. Gerritsen

Promotor: T.H. Oosterkamp

We explore the possibilities to combine magnetic resonance techniques with atomic force microscopy together in a single microscope: the MRI-AFM, also called Magnetic Resonance Force Microscopy (MRFM).

High throughput microscopy

Establishing human induced pluripotent stem cell (iPSC) CRISPR-Cas9-based fluorescent reporter cell lines for NF-kB signalling. We will use this to enable studying the dynamic activation and downstream signalling of Toll-like receptor and cytokine signalling in iPSC-derived differentiated cells in a…

The purpose of this study was to investigate the formation and growth kinetics of complexes between proteins and oppositely charged polyelectrolytes.

Promotor: B. van de Water

Promotor: B. van de Water

The Netherlands Organisation for Scientific Research (NWO) has made a subsidy of 17 million euros available to further develop a Netherlands network for electron microscopy (NEMI). The network comprises five UMCs and eight universities, with Utrecht in the coordinating role. From Leiden, the Institute…

Moving an electron microscope of 2000 kg is a delicate challenge. The highly sensitive instrument needed to be moved to a new measurement hall, but even a tiny bump could damage it. After a few nerve-racking weeks of preparing the move and reinstalment, the researchers finally have a verdict: the instrument…

In this thesis the combinatorial use of bioorthogonal labelling and Electron Microscopy (EM)-based imaging techniques is explored to enable observations of specific molecular targets in their ultrastructural context within the cell.

From surface science towards industrial practice using high-pressure scanning tunneling microscopy.

Chinese and Leiden scientists came together in Leiden to study the intricacies of modern visual techniques.