Promotor: Prof.dr. J. Kuiper, Co-Promotor: S.C.A. de Jager

Glucocorticoids (GCs) are widely prescribed as anti-inflammatory drugs due to their well-established immunosuppressive effects.

A new therapy for the serious Graft-versus-Host disease

The Immune Activation and Tolerance group is headed by Dr. Bram Slütter. Vaccination is an experimental, but promising, treatment strategy for atherosclerosis. Previous work has shown that immunization of mice with modified LDL particles can reduce atherosclerotic lesion development, however such vaccines…

Cardiovascular syndromes are the major cause of death in Western societies.

Traditionally, vaccines are administered intramuscularly using conventional hypodermic needles, which cause pain and distress. Microneedles are very short needles (smaller than 1 mm) that are practically invisible to the naked eye.

Promotores: Prof.dr. J. Kuiper, Prof.dr. P.H.A. Quax

Worldwide resurgence of pertussis necessitates the need for improvement of pertussis vaccines and vaccination strategies.

Our immune system protects us against disease, but every now and then, something goes wrong: an enemy invades our bodies or our immune system attacks our own cells and we become ill. Doctors and researchers at the Leiden University Medical Center (LUMC) want to be able to manipulate the immune system…

Promotor: Prof.dr. H.P. Spaink, Co-promotor: Prof.dr. A.H. Meijer

The LUMC has a longstanding tradition researching the role of the immune system in infectious diseases, transplantation and auto-immune diseases.

The Aging and Immunity group is led by Dr. Amanda Foks. In this group we aim to investigate how aged immune cells contribute to atherosclerosis and identify novel therapeutic targets and strategies to extend health span and inhibit cardiovascular disease.

The potential of bioneedles to deliver influenza vaccines was investigated.

What are the host immune defence mechanisms that control intracellular infections and how are these subverted by pathogens?

Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn, Co-promotor: Dr. M.J.M. Schaaf

In this research project, we aimed to obtain more insight in the role of mast cells in the immune driven disorders rheumatoid arthritis and atherosclerosis.

Can small molecule drugs restore the immune system’s ability to fight tuberculosis by boosting autophagy?

One of the main challenges in the development of an effective anti-cancer vaccine is the generation of an adequate and directed cellular immune response.

This thesis focuses on important life history traits and their trade-offs using two hexapod models. Particularly, the trade-off between immune defense and embryonic developmental speed is a central theme of this study.

Poly(lactic-co-glycolic acid) (PLGA) particles have been extensively studied as biodegradable delivery system to improve the potency and safety of protein-based vaccines. In this study we analyzed how the size of PLGA particles, and hence their ability to be engulfed by dendritic cells (DC), affects…

Nanoparticles can be used as delivery systems for both small molecules and macromolecules such as proteins, peptides or oligonucleotides. This thesis focuses on the use of liposomes, nanometric vesicles formed by a lipid bilayer enclosing an aqueous core. Liposomes are highly versatile delivery syst…

Promotor: G.F.A. Kersten, W. Jiskoot, Co-promotor: B. Metz

Promotor: Prof.dr. J. Kuiper

In the hunt for a vaccine against cervical cancer, fundamental knowledge about the immune system and organic chemistry have been brought together and have already resulted in a vaccine that is now being tested in clinical trials. Scientists are now working hard on an improved variant.

In autoimmune diseases such as rheumatism and diabetes the immune system attacks autologous proteins. Leiden researchers are trying to discover how this comes about.

Promotores: J. Jonkers, K.E. de Visser

To what extent and in what manner can experimental video be used to reclaim the capacity for telling one’s own story, on one's own terms, speaking in tongues outside the linguistic framework of immune system discourse?

Cardiovascular diseases are among the most frequent causes of death in the world. The main underlying pathology of cardiovascular diseases is the development of atherosclerosis in the medium and large-sized arteries.

Promotores: T. Hankemeier; R. Berger, Co-promotor: R.J. Vreeken

Cardiovascular diseases are the primary cause of death in the world with atherosclerosis as primary underlying cause.

The cellular metabolism of immune cells constitutes an important part of their proper function and activation. Using T cells as an example, this phenomenon is highlighted by the metabolic reprogramming that occurs in these cells upon activation.

This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.

This work describes the use of click-to-release chemistry to get spatiotemporal control over immunocytokine activity. Until now, immunocytokines (cytokines coupled to a tumor-targeting-moiety) remained active throughout the body, being able to bind their respective receptors, causing mild to severe…

Healthy aging is one of the prime goals in today's society and atherosclerosis is among the greatest causes of morbidity in elderly. Cardiovascular disease patients receiving treatment are often of advanced aged and have an aged immune system, which limits translating experimental findings to the pa…

This thesis advances our understanding of lipid uptake, a vital first step in lipid metabolism, by developing innovative click chemistry-based tools to study how immune cells internalize lipids.

Cationic peptide amphiphile nanoparticles are employed for co-delivery of immune modulator CpG and antigen. This results in better targeting to the antigen presenting cells and eliciting strong Th1 response, which is effective against the intracellular pathogens.

The major aim is to determine the psychophysiological responses to stress and stress-management interventions and to examine the role of stress and stress-related psychophysiological mechanisms (e.g., cognitions, behaviors, and physiological stress responses) in both healthy and medical populations.

Tuberculosis bacteria and other intracellular pathogens use cells of our immune system as Trojan horses to spread into tissues. Annemarie Meijer, professor of immunobiology, explains how research into innate defence mechanisms using zebrafish inspires novel strategies for infectious disease treatmen…

PhD defence

Promotor: H.P. Spaink, A.H. Meijer Co-promotor: R. Marin-Juez

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

We showed that constitutive and inducible chemical and morphological defenses against Western flower thrips differ between tomato and chrysanthemum plants.

Promotor: Prof.dr. J.N. Kok

A new treatment for atherosclerosis showed promising results in isolated cells but proved to be less effective in initial animal tests. Bachelor student Biopharmaceutical Sciences Willemijn van der Heijden aimed to understand why. She investigated whether the formation of a protein layer around the…

The aim of this work was to study the applicability of antigen-coated pH-sensitive microneedle arrays for effective vaccination strategies. Therefore, a model antigen (ovalbumin) was coated onto pH-sensitive (pyridine-modified) microneedle arrays to test pH-triggered antigen release by applying the…

Seasonal influenza vaccines provide protection against matching influenza A virus (IAV) strains mainly through the induction of neutralizing serum IgG antibodies. However, these antibodies fail to confer a protective effect against mismatched IAV. This lack of efficacy against heterologous influenza…

This thesis describes the synthesis and biological evaluation of TLR2/6, TLR4, TLR7/8 and TLR9 ligands, of which the activity can be conditionally controlled.

New drugs for use as tuberculosis (TB) treatment are needed due to the constrains of classical antibiotics against TB and the rise of antibiotic-resistant strains, making TB a harder and harder disease to treat.

Atherosclerosis is a progressive disease resulting in the formation of an arterial plaque. Despite lipid lowering, recurrent cardiovascular events remain a risk. While atherosclerosis is primarily lipid-driven, the immune system plays a critical role in the pathophysiology.

PhD defence