Promotor: Prof.dr. J.N. Kok

How do pathogenic mycobacteria alter lipid metabolism in human cells and patients, and which disrupted pathways could be targeted for new antibiotics?

Promotor: Prof.dr. H.P. Spaink, Co-promotor: Prof.dr. A.H. Meijer

The function of TLRs in innate immunity has aroused worldwide attention soon after its discovery. Because of the broad functions of TLR2 in innate immunity, the drive for the development of TLR2-targeted vaccines or therapeutic treatments has accelerated in the last decades.

The effective treatment of tuberculosis (TB) remains a major challenge to global health.

The LUMC has a longstanding tradition researching the role of the immune system in infectious diseases, transplantation and auto-immune diseases.

Our immune system protects us against disease, but every now and then, something goes wrong: an enemy invades our bodies or our immune system attacks our own cells and we become ill. Doctors and researchers at the Leiden University Medical Center (LUMC) want to be able to manipulate the immune system…

How do lipases in Mycobacterium tuberculosis help the bacteria survive, and can we target them to create new antibiotics?

Promotor: Prof.dr. H.P. Spaink, Co-promotor: Dr. M. van der Zee

Autophagy is a fundamental degradative process, maintaining cellular homeostasis and functions in host defense against intracellular pathogens, including mycobacteria and Salmonella.

Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn, Co-promotor: Dr. M.J.M. Schaaf

Worldwide resurgence of pertussis necessitates the need for improvement of pertussis vaccines and vaccination strategies.

Promotores: A.H. Meijer, H.P. Spaink

The research described in this Thesis was aimed at designing and synthesizing nature-inspired compounds as part of TB vaccine discovery.

Our research aims to develop drugs that boost the host immune response to better defend us against mycobacteria.

This thesis focuses on the role of chemokine receptors CXCR3 and CCR2 in the inflammatory process and infection control using the zebrafish model.

Mycobacterium tuberculosis (Mtb), the pathogen causing tuberculosis (TB), is the leader among all pathogens responsible for the most human deaths today and it is considered as one of the top 10 causes of death worldwide. There is an increasing occurrence of multidrug-resistant and even totally drug-resistant…

β-Lactamases are enzymes that can break down β-lactam substrates, such as antibiotics, preventing the use of these antibiotics for the treatment of various infectious diseases. However, some compounds, β-lactamase inhibitors, can block these enzymes allowing for possible treatments using a combination…

Glucocorticoids (GCs) are widely prescribed as anti-inflammatory drugs due to their well-established immunosuppressive effects.

Josien de Klerk and Tennessee Miller examined the interplay between students’ illness and recovery experiences and academic work culture in this context.

Promotor: Prof.dr. H.P. Spaink, Co-promotor: Prof. dr. A.H. Meijer

Enzymes are innately sensitive to changes in the amino acid sequence, which largely constrains their evolutionary potential, i.e., evolvability. This evolutionary burden can be alleviated in the presence of stabilizing mutations, which increase the buffering capacity of enzymes to tolerate mutations…

Michiel van der Vaart with a team from Leiden University and the LUMC, led by IBL-researcher Annemarie Meijer, discovered that DRAM1 is a protein that regulates anti-bacterial autophagy, a defense mechanism against infections such as tuberculosis.

NVIC is delighted to host Dr. Marina de Regt from the Department of Social and Cultural Anthropology of Vrije Universiteit Amsterdam as our NWIB Visiting Professor during 2,5 months this Fall.

IBL-researcher Phil Elks in the group of Annemarie Meijer won the Cell Observatory Publication of the Year 2013. He received the award for his paper in PLoS Pathogens, showing that activation of hypoxia signaling protects against mycobacterial infection.

This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.

PhD defence

BlaC is the β-lactamase of Mycobacterium tuberculosis. We show that it can recover from inhibition by clavulanic acid and that phosphate helps it do so.

Once it was considered the Switzerland of the Middle East, but today Lebanon is a country deeply affected by the economic crisis, political stalemate and an international context of growing tensions.

Promotor: H.P. Spaink, A.H. Meijer Co-promotor: R. Marin-Juez

The cellular recycling system in zebrafish is capable of eating harmful bacteria and thus resist infections such as tuberculosis and salmonellosis. That is written by Leiden biologists from the group of Annemarie Meijer. Stimulating this form of defence could be used in new treatment methods against…

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

The infection rate is rising again in the Netherlands, which means it may also be rising among Leiden University’s students and staff. How is the University responding? And what dilemmas is it facing? We spoke to our Rector Magnificus, Chief Security Officer and two other administrators.

The opportunistic bacterium Burkholderia cenocepacia is feared by cystic fibrosis patients and is emerging in hospital-acquired infections. An international study sheds new light on the infection mechanism of this opportunistic pathogen that may have large implications for treatment strategies.

Joost Beltman (LACDR, Leiden University) has provided novel insights in the way T cells migrate towards virus-infected cells. This was accomplished by a combination of experimental research in the group of Ton Schumacher (Dutch Cancer Institute, NKI) and computer simulations in collaboration with Rob…

Can small molecule drugs restore the immune system’s ability to fight tuberculosis by boosting autophagy?

PhD defence

Promotores: T. Hankemeier; R. Berger, Co-promotor: R.J. Vreeken

Tuberculosis causes 1.5 million deaths yearly and anti-tuberculosis therapies are threatened by emergence of drug resistance. Development of innovative drug combinations should be accelerated with the use of translational pharmacological models. Moreover, host-directed therapies (HDT), which stimulate…

The research described in this thesis has, using the zebrafish as a model system, shed new light on the intricate relationship between TB and DM2, in particular on the role of leptin, SHP-1 and glucocorticoids.

Antimicrobial drugs constitute a fundamental part of modern medicine. The global rise in antimicrobial resistance poses a major threat to global health.