This article researches the public perception of terrorist attacks by measuring the importance the public assigns to attributes of terrorist attacks.

The research described in this thesis shows that hypercholesterolemia, a well-established risk factor for atherosclerosis, can impact skin lipid pool and barrier function already at young age. In the field of atherosclerosis, we showed that the small peptide Lyp-1 can be used as a targeting molecule…

Promotor: Prof.dr. T. Hankemeier, Prof.dr. J.C.T. Eijkel (Twente University)

Polymyxins are clinically used antibiotics, discovered in mid-20th century. Once abandoned due to excessive nephrotoxicity, they are now used increasingly to address infections caused by multi-drug resistant Gram-negative bacteria.In this thesis, we describe the development and synthesis of analogues…

During post-embryonic development into adults, animals face an environment that fluctuates constantly. For example, in nutrient availability, temperature, and osmolarity (e.g., salt concentration).

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea

Promotor: Prof.dr. T. Hankemeier, Co-promotor: Dr. Theo Reijmers

Key publications of the Skin Barrier and Vaccination group.

Promotor: Prof.dr. R. Verpoorte, , Co-Promotor: Young Hae Choi

Streptomyces are filamentous bacteria that produce more than two-thirds of known antibiotics.

This thesis focuses on important life history traits and their trade-offs using two hexapod models. Particularly, the trade-off between immune defense and embryonic developmental speed is a central theme of this study.

Salicylic acid (SA) is a plant hormone, which is involved in the defense responses to pathogens and to abiotic stress, and in the regulation of plant growth and development.

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

Metabolomics group, LCQUI, Universidade UENF, Campos dos Goytacazes/Rio de Janeiro - Brazil

Leiden University is an international, broad-based university. We distinguish five core domains for our research and teaching activities.

Supervisor: Gerard van Westen

Plant exudates appeared long time ago in the history line of life in the early Devonian. They are typical saps produced by specialized cells.

Source: Eur J Med Chem, Volume 101, pp. 681-91 (2015)

My PhD project studies how the temporally sequential Hox gene expression is regulated during head-tail patterning of the frog embryo.

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

The objective of this study was to investigate the expression and function of GRHL2 in different breast cancer subtypes.

Chemical-induced organ toxicity is a major concern in the development and use of chemicals, including drugs, pesticides, industrial chemicals and cosmetics. In the last decades, the onset and progression of chemical-induced organ toxicity has been linked amongst others to perturbation of mitochondri…

Promotores: H. Schiessel, G.T. Barkema

The zebrafish is a promising vertebrate model organism in early drug discovery and development.

One of the main challenges in the development of an effective anti-cancer vaccine is the generation of an adequate and directed cellular immune response.

Inactivating mutations in BRCA1 or BRCA2 genes predispose to several types of cancer.

Immunotherapies for cancer are an emerging class of therapeutic strategies which aim to treat cancer via augmentation of the immune system.

The stratum corneum is the outermost skin layer and consists of dead cells embedded in a lipid matrix.

This paper describes two synthetic lipid models designed to replace human stratum corneum (SC) in studies of the impact of volatile organic chemicals on the molecular organization of the skin barrier lipids.

The mission of the Plant Science research programme is to contribute to the sustainable production of high quality crops, flowers and high-value bio-based products. This is realised by generating fundamental knowledge of basic biological processes related to development of plants and their interaction…

The lipid membrane is a basic structural component of all living cells. Embedded in this nanometer-thin barrier, membrane proteins shape the membrane and at the same time respond to the shape of the membrane.

There is considerable need for improved skin models to develop safe and efficient drugs for skin delivery as well as for cosmetic and chemical product testing on the skin.

PURPOSE: The aim of the study was to develop a cheap and fast method to produce hollow microneedles and an applicator for injecting vaccines into the skin at a pre-defined depth and test the applicability of the system for dermal polio vaccination.

Promotor: Prof.dr. A. P. IJzerman, Co-Promotor: Dr. L.H. Heitman

Promotor: Prof.dr. J. Brouwer, Co-promotor: Dr. Remus Th. Dame

Promotor: Prof.dr. H.P. Spaink, Co-promotor: Dr. M. van der Zee

Promotor: Prof.dr. R. Verpoorte, Co-promotores: Dr. Y.H.Choi, N.R. Mustafa

The long-held desire - to link structure directly to function and to explain molecular mechanisms based on basic chemical or physical principles - is finally coming closer, satisfying not only our scientific curiosity but also offering new solutions to the many challenges in the field of health, energy…

How do mycobacteria subvert the defenses of host immune cells?

Natural vanilla flavor is one of the most important in the world. However, the cost of this flavor is expensive. Production of this flavor by alternative methods, could reduce the cost.

The electrochemical oxygen reduction reaction (ORR) is an essential half-reaction for the utilization of hydrogen as a sustainable fuel, via the conversion of hydrogen to electrons and protons facilitated by the ORR. In the most common fuel cells, the ORR is requires high loadings of non-abundant platinum…

Can we link multiple components from herbal extracts with their biological activities?

The main goal of the research described in this thesis was the development of new photoactivated chemotherapy (PACT) ruthenium(II) complexes bearing a non-toxic photolabile ligand.

Promotor: Prof.dr. B. van de Water, Co-promotores: L.T.M. van der Ven, A.S. Kienhuis

Animal models suggest that the chemokine ligand 2/CC-chemokine receptor 2 (CCL2/CCR2) axis plays an important role in the development of inflammatory diseases. However, CCR2 antagonists have failed in clinical trials because of a lack of efficacy.

A main goal of MSBB research is to address the major bottle-necks in combatting viral diseases: the lack of experimental models that are representative of human pathology and are cost effective and versatile. Viral diseases such as those that cause viral hemorrhagic syndromes or severe acute respiratory…

A complex community of microbes develops in the infant gut shortly after birth. We call this community the infant gut microbiota. The microbiota influences the health of the infant, which makes the composition and function of the infant gut microbiota an important topic to study.

Antibiotic resistance, caused by widespread use of antibiotics, leads to bacterial infections that are difficult, if not impossible, to treat and is a major worldwide health concern.

This thesis describes the structural and biochemical characterization of the β-lactamase BlaC from Mycobacterium tuberculosis (Mtb), and the Alr and YlmE proteins from Streptomyces coelicolor A3(2).Mtb is the main cause of tuberculosis.

Promotor: T. Schmidt, Co-Promotor: B.E. Snaar-Jagalska