Accurate prediction of the unbound drug concentration-time profile at the CNS target site is crucial for the assessment of the right drug concentration-effect relationship. PBPK models have supported the PK prediction of the CNS target sites and the translation of PK data between species and between…

Analysis of drug adversity

We have developed a microfluidic platform for complex 3D organotypic cell cultures (so-called organ-on-a-chip systems) which are suited for integration in 96 or higher well plate format.

The currently available in vitro generated human skin equivalents resemble the human skin in many aspects. However, some essential barrier characteristics do not fully mimic the native barrier. Consequently, the human skin equivalents cannot be used for screening of drugs for skin penetration.

This thesis investigates how non-genetic factors, such as inflammation and concomitant medication, impact hepatic drug metabolism and subsequent drug metabolizing phenotype predictions.

In the literature, several types of microneedles have been extensively described. However, porous microneedle arrays only received minimal attention. Hence, only little is known about drug delivery via these microneedles. However, porous microneedle arrays may have potential for future microneedle-based…

Research in the BioTherapeutics research programme is aimed at a smoother and speedier transition from preclinical research to pharmacological interventions, and the delivery of drugs in the human body by use of small molecules and biologics.

The advanced insights obtained for the CNS PBPK model development are currently used to develop advanced mathematical models for drug distribution prediction in other body tissues protected by barriers, such as the gut. The gut PBPK model will be linked to drug effects for treatment of colon cancer.

The impact of the phenotypic plasticity of P. aeruginosa and the distinct CF lung microenvironments on antibiotic drug effects and resistance remains poorly understood.

Source: ChemMedChem (2015)

For more information regarding ongoing research projects, please contact Prof. Johan Kuiper.

In this PhD thesis, available data on the PK and/or PD of different targeted therapies, chemotherapy and immunotherapies obtained during both routine clinical practice and prospective clinical studies were analyzed.

Papyrology+, founded in 2014, is a collaboration of Leiden scholars studying (Abnormal) Hieratic, Demotic, Aramaic, Greek, Latin, Coptic, and Arabic papyri from a socio-historical, economic and linguistic perspective. Papyrology+ aims to explore new opportunities and directions in the study of ancient…

University building Campus Spui The Hague

Political Science: National and International Politics is a broad study with a lot of depth. You will study political issues at the local, national and international levels, from a variety of perspectives. You will be taught by renowned, committed professors. In Leiden you will find a real student city…

The Hazelhoff Centre for Financial Law team regularly publishes in both Dutch and international academic journals and book series.

Kyra van der Moezel defended her thesis on 7 September 2016.

Contact information & Visitors address

In CCLS several subgroups have formed, below you can find an overview of these groups with the names of the leading researchers and a short outline of the project.

To address major societal challenges, research needs to go beyond the boundaries of traditional disciplines. We believe this starts with education. By exposing students to people from different scientific fields, they learn to deal with diverse perspectives and can broaden their horizons. Professors…

Overview of Leiden publications on Central Asia. For additional publications dedicated to a single country, please go to individual pages of the researchers, which you can access through the Researchers page.

One of the recurring points of discussion within the Criminal Justice research programme is the question of what behaviour should or should not fall within the scope of criminal law. This research theme is examined from a legal-dogmatic and social-scientific perspective.

An overview of awards and prizes granted to our staff and students in 2021, as well as special appointments at Leiden University and other institutions.

The aim of the project is two-fold: first, to further scholarly understanding of the human dynamics that shaped the growth of Dorestad in a broader, international context; and second, to test the potential of multi-isotopic analyses on an early medieval Dutch assemblage.

This thesis explored the molecular pharmacological mechanisms of targeting CB2R via investigation of novel drug discovery concepts such as target binding kinetics, allosteric modulation and biased signaling.

Promotores: Prof. Dr. Th.J.C. van Berkel, Prof. Dr. M. van Eck

Promotores: Prof.dr. G.F.A Kersten, Prof dr. J.A. Bouwstra

This thesis focuses on data found in the field of computational drug discovery. New insight can be obtained by applying machine learning in various ways and in a variety of domains. Two studies delved into the application of proteochemometrics (PCM), a machine learning technique that can be used to…

Light obscuration (LO) is the current standard technique for subvisible particle analysis in the quality control of parenterally administered drugs, including therapeutic proteins.

Cardiovascular diseases are still a major concern for the global health.

The effectiveness of the skin barrier underlies the outer layer of the skin: the stratum corneum (SC). However, in several skin diseases this barrier is impaired. In two inflammatory skin diseases, atopic eczema and Netherton syndrome, an increased level of monounsaturated fatty acids (MUFAs) has been…

High throughput microscopy

Drug-target binding kinetics determine the time course of the central event in pharmacotherapy: Drug-target interaction.

Oxidation is a common degradation pathway that affects therapeutic proteins and peptides during production, purification, formulation, transportation, storage and handling of solid and liquid preparations. In the present work we review the scientific literature about structural and biological consequences…

Pericytes, the mural cells of blood microvessels, are important regulators of vascular morphogenesis and function that have been postulated to mechanically control microvascular diameter through as yet unknown mechanisms.

Lipid signaling is an essential biological event/process in a plethora of pathophysiological conditions. The underlying idea of this thesis is that many of the roles and the complex interplay of the individual signaling lipids in inflammatory processes and related conditions in health and disease is…

The objective is to develop in vitro antigen degradation assays in order to mimic the fate of the antigen in vivo.

With an emphasis on developing novel miktoarm star polymers termed PeptoMiktoStars, this thesis leverages asymmetric polymeric polypept(o)ide frameworks to advance their effectiveness in delivering hydrophobic drugs and nucleic acids.

In this thesis, we aim to shed light on the diverse and often opposing roles of integrin α3β1 in cancer.

Projects within the theme ‘Computational Biology’ aim to employ mathematical or computational dynamical modelling approaches to discover novel concepts that are important for drug development.

Atopic eczema is a skin disease with increasing incidence currently affecting 25% of the paediatric population. In atopic eczema the skin barrier function is reduced. This barrier is formed by the outermost layer of the skin called the stratum corneum (SC). The SC consists of terminally differentiated…

In this thesis, we developed new ways to use a technique called electroextraction (EE) to extract charged molecules from one liquid into another using an electric field.

This thesis describes the importance of being able to control the selectivity of potential drug candidates.

The predication of the effect (efficiency and toxicity) of a drug in a patient is very important in (i) clinical decision support and (ii) the development of novel drug treatments.

Another research line is the development of a spatial CNS drug distribution model, by ultimately including the 3-dimensional anatomical organization of the CNS.

We are developing biology-driven platforms to measure metabolites based on liquid chromatography-mass spectrometry (LC-MS), gas chromatography-MS (GC-MS), and capillary electrophoresis-MS (CE-MS).

The predication of the effect (efficiency and toxicity) of a drug in a patient is very important in (i) clinical decision support and (ii) the development of novel drug treatments. We apply our technology and methods to find biomarkers for key disease pathways and toxicity in clinical studies and in…

Main challenges in skin vaccination are overcoming the stratum corneum (SC) barrier and targeting the antigen presenting cells (APC) in the epidermis and the dermis.

The aim of this research area is to be able to predict human drug response on the basis of mathematical models that are developed using preclinical experiments and prior knowledge.